Inclined settler

0.5–3000

20, 50

3000

Yes

>30 min

No

Cooling loop,

peristaltic

pump

CHO,

HEK293,

AGE1.CR,

BHK21,

Hybridoma

IAV

[ 62]

Acoustic filter

0.5–200

50

1000

Yes

>10 min

No

Cooling,

controller,

peristaltic

pumps

HEK293,

AGE1.CR

IAV, MVA,

Ad5, LV

[ 41, 48– 50]

Hydrocyclone

5–500

50

2200

Yes

0.03–0.1 s

No

Peristaltic

pump

CHO

No

Centrifuge

1–1000

30

3700

Yes

2–9 min

Yes

Peristaltic

pump

Hybridoma,

CHO

No

White fields: Membrane-based/separation by size (PBBR use of macrocarriers for cell attachment); grey fields: separation by sedimentation. HFBR: Hollow-fiber bioreactor,

PBBR: packed-bed bioreactor, TFF: tangential flow filtration, ATF: alternating tangential flow filtration, CHO: Chinese hamster ovary cells, MDCK: Madin Darby canine

kidney cells, HEK293: human embryonic kidney cells, Huh7.5: human hepatocellular carcinoma cells, AGE1.CR: avian duck cells, IAV: influenza A virus, CHIKV:

chikungunya virus, HIV: human immunodeficiency virus, HAV: hepatitis A virus, HCV: hepatitis C virus, MVA: modified vaccinia virus Ankara, ZIKV: Zika virus, YFV:

yellow fever virus, IPV: inactivated Polio virus, AD5: adenovirus-5, LV: lentivirus; RABV: rabies virus, n.a.: not applicable. Adapted from [ 17,19,43,52,53,63].

Homepages of Fibercell systems, Sartorius, Repligen, and other manufacturers.

Process intensification

151